Regulatory

Part:BBa_K2275006:Design

Designed by: I-Hsin Chen   Group: iGEM17_NCKU_Tainan   (2017-10-09)


PyeaR(BBa_K216005) with additional NsrR binding site behind B0030 RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

We found the sequence of NsrR binding site in E. coli, and we designed the couple of primer that attached NsrR binding site sequence for construction.


References