Regulatory
Part:BBa_K2275006:Design
Designed by: I-Hsin Chen Group: iGEM17_NCKU_Tainan (2017-10-09)
PyeaR(BBa_K216005) with additional NsrR binding site behind B0030 RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 126
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
We found the sequence of NsrR binding site in E. coli, and we designed the couple of primer that attached NsrR binding site sequence for construction.